BioQuick Hot Start Taq DNA Polymerase V1
This product is a novel antibody-coated hot-start enzyme developed by our company. The coated antibody rapidly deactivates at 95°C within 1 minute, allowing Taq enzyme to regain activity. This feature significantly improves the suppression of nonspecific reactions caused by primer mis-annealing or primer-dimers during the PCR system setup and amplification process, resulting in excellent performance in multiple amplifications. The product possesses strong resistance to inhibitors, making it particularly suitable for samples with a high presence of inhibitors, which are challenging for regular Taq enzymes to amplify.
Product Description
Taq DNA polymerase V1 is a novel antibody blocked and heat-activated enzyme. The blocked antibody is rapidly inactivated at 70℃ for 1 minute, and the Taq enzyme is reactivated.The enzyme can better inhibit the non-specific reaction caused by the non-specific annealing of the primers or the primer polymers during the preparation and amplification of PCR system, so that the product can achieve a good effect in multiple amplification. The enzyme has a high elongation rate, under general conditions, the elongation rate is 5~15 seconds /1000bp, while the amplification is less than 1000bp, the elongation rate can reach 2 seconds /1000bp. At the same time, the enzyme has a strong inhibitor resistance, and it is especially suitable for rapid amplification of the samples with many inhibitors, which are difficult to be amplified by ordinary Taq enzyme, and can save time and cost. The specially modulated buffer is used to make it more sensitive to very low concentration templates, so as to achieve higher yield and obtain stable and reliable research results.
Product Features
Fast Amplification Speed: 2 s/kb. On Bioer's machine, DNA detection can be completed in 25 minutes, and RNA detection in 35 minutes (45 cycles).
Batch Quality Stability: Low concentrations of BioReady Hot Start Taq produce normal bands during amplification.
Wide range of amplicon length (up to kb): ＜6Kb
Amplification Speed: 5-15 sec/Kb (General Condition), 2 sec/Kb (＜1000bp)
Application Field
Polymerase Chain Reaction (PCR)
Site-Directed Mutagenesis
Cloning
Sequencing
Genotyping
Gene Expression Analysis
Application Cases
The experiment involved the gradient dilution of African Swine Fever Virus (ASFV) quality control samples. A comparison of amplification performance was conducted using BioQuick Hot Start Taq DNA Polymerase V1 and T brand. The results show that BioQuick Hot Start Taq DNA Polymerase V1 achieved a lower Ct value compared to the T brand reagent, and it remained detectable even in low-concentration samples. This indicates that its amplification performance and detection sensitivity are superior to the T brand reagent.
Storage Conditions
-25~-15℃, protected from light, valid for 12 months.
Ordering Information
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