BioReady Heat-labile UDG
BioReady Thermosensitive Uracil-DNA Glycosylase (UDG) is derived from recombinant cloning and expression in Escherichia coli. It effectively hydrolyzes uracil (U) in single-stranded or double-stranded DNA longer than 6 base pairs, with no activity on RNA. Its primary application is to prevent contamination of PCR amplification products. It works by replacing dTTP with dUTP in the PCR reaction, creating PCR amplification products containing dU bases. UDG then cleaves the dU bases in these amplification products, creating abasic sites, preventing the DNA strand from serving as a template for subsequent PCR, thus achieving the goal of preventing PCR product contamination. Additionally, this thermosensitive UDG becomes inactive in the subsequent 50°C reverse transcription or 95°C pre-denaturation steps without affecting the amplification of the template.
Product Description
BioReady Heat-labile UDG（Uracil-DNA Glycosylase）catalyzes the release of free uracil from longer then 6bp dU-containing single-stranded or double-stranded DNA whereas RNA and normal dT-containing DNA. UDG excises uracil form dU-containing PCR product when Dttp replace with dUTP was incorporated into DNA. The resulting abasic sites are blocked from replication by PCR. It mainly used to prevent contamination of PCR amplification products. This enzyme is sensitive to heat. It can be completely and irreversibly inactivated after 10 min at temperatures above 50°C.
Product Features
Thermosensitive, heat inactivated at 50°C for 10 minutes.
Wide applicability, compatible with one-step RT-PCR systems.
Application Field
Polymerase Chain Reaction (PCR)
Site-Directed Mutagenesis
Cloning
Sequencing
Genotyping
Gene Expression Analysis
Application Cases
Experiments were conducted using one-step RT-qPCR reagents with and without BioReady Thermosensitive Uracil-DNA Glycosylase (BSA51) to evaluate the amplification of templates containing uracil (U) after digestion
at 25°C for 2 minutes.
Results:The results demonstrate that BioReady Thermosensitive Uracil-DNA Glycosylase (UDG) effectively degrades PCR products containing uracil (U). In the presence of PCR products containing U, the one-step RT-qPCR without UDG had an average amplification Ct of 29.66, while the one-step RT-qPCR with the inclusion of thermosensitive UDG showed no amplification at all.
Storage Conditions
1. The kit needs to be shipped refrigerated.
2. Kit storage: Store in a -20°C refrigerator away from light, and the repeated freezing and
thawing should not more than 7 times.
3. Validity period: 12 months, please use within the validity period.
Ordering Information
Related Products
You May Also Like
BioRT All-in-One RT Master Mix for qPCR（+gDNA Wiper）
This kit is a new-generation qPCR-specific reverse transcription kit. The product integrates all components required for genomic DNA removal and RNA reverse transcription into a single tube. Simply add template and water to initiate the reaction, which can be completed in as little as 5 minutes. The resulting cDNA is compatible with both dye-based and probe-based qPCR methods.
BioRT Real Time RT-PCR Kit（SYBR Green）
The BioRT Real Time RT-PCR Kit (SYBR Green) is designed for efficient one-step fluorescent quantitative PCR detection using RNA as the template. This kit enables precise and reliable real-time monitoring of PCR amplification with SYBR Green dye, which binds specifically to double-stranded DNA. It offers a streamlined workflow, making it ideal for gene expression analysis, viral RNA detection, and other RNA-based research applications. The kit provides high sensitivity and specificity, ensuring accurate quantification of target genes with minimal preparation time.
BioEasy Universal qPCR Master Mix (SYBR Green)
This kit utilizes nucleic acid amplification technology combined with SYBR Green I fluorescent dye for Real-Time PCR, enabling rapid and accurate detection and quantification of target genes. The kit provides a 2× Universal SYBR Green Mix, requiring only the addition of primers and template to perform Real-Time PCR reactions, making it fast and easy to operate. Through improvements in the reaction components and the application of high-quality fast enzymes, this kit offers high amplification efficiency, strong specificity and fast reaction speed. The reaction and quantification efficiency for high GC sequences has been significantly enhanced. Additionally, the kit has optimized ROX dye in the system, allowing compatibility with various ROX versions of PCR instruments while greatly improving sensitivity.