BioReady rTaq
rTaq is a modified form of the Taq DNA polymerase enzyme that is commonly used in molecular biology applications, particularly in the polymerase chain reaction (PCR) technique. The "r" in rTaq stands for "recombinant," indicating that it is produced using recombinant DNA technology. This modified enzyme exhibits improved stability at higher temperatures compared to the native Taq polymerase, allowing for more efficient DNA amplification in PCR reactions. rTaq is widely utilized in various research and diagnostic settings due to its robust performance, high fidelity, and versatility in amplifying target DNA sequences.
Product Description
This product is a heat-resistant DNA polymerase with a molecular weight of 94 kDa. It has undergone specific mutagenesis to reduce its dependence on activators and enhance amplification efficiency. It is particularly suitable for DNA amplification of fragments smaller than 6 kb, DNA labeling, DNA sequencing, etc. This polymerase possesses 5’→3’ exonuclease activity but lacks 3’→5’ exonuclease activity. The amplification products have a 3’-dA overhang, making them directly usable for TA cloning.
Product Features
Low Host gDNA Residue: Unique purification process, purity >95%, with no nucleases remaining.
High Sensitivity: Detection sensitivity can reach 1ng.
Wide Template Range: Suitable for amplifying templates of different lengths up to 6kb.
Consistent Batch Quality: Stringent production processes and quality control standards result in minimal batch-to-batch variation.
Application Field
Polymerase Chain Reaction (PCR)
Site-Directed Mutagenesis
Cloning
Sequencing
Genotyping
Gene Expression Analysis
Storage Conditions
-20 ℃
Ordering Information
Related Products
You May Also Like
BioRT All-in-One RT Master Mix for qPCR（+gDNA Wiper）
This kit is a new-generation qPCR-specific reverse transcription kit. The product integrates all components required for genomic DNA removal and RNA reverse transcription into a single tube. Simply add template and water to initiate the reaction, which can be completed in as little as 5 minutes. The resulting cDNA is compatible with both dye-based and probe-based qPCR methods.
BioRT Real Time RT-PCR Kit（SYBR Green）
The BioRT Real Time RT-PCR Kit (SYBR Green) is designed for efficient one-step fluorescent quantitative PCR detection using RNA as the template. This kit enables precise and reliable real-time monitoring of PCR amplification with SYBR Green dye, which binds specifically to double-stranded DNA. It offers a streamlined workflow, making it ideal for gene expression analysis, viral RNA detection, and other RNA-based research applications. The kit provides high sensitivity and specificity, ensuring accurate quantification of target genes with minimal preparation time.
BioEasy Universal qPCR Master Mix (SYBR Green)
This kit utilizes nucleic acid amplification technology combined with SYBR Green I fluorescent dye for Real-Time PCR, enabling rapid and accurate detection and quantification of target genes. The kit provides a 2× Universal SYBR Green Mix, requiring only the addition of primers and template to perform Real-Time PCR reactions, making it fast and easy to operate. Through improvements in the reaction components and the application of high-quality fast enzymes, this kit offers high amplification efficiency, strong specificity and fast reaction speed. The reaction and quantification efficiency for high GC sequences has been significantly enhanced. Additionally, the kit has optimized ROX dye in the system, allowing compatibility with various ROX versions of PCR instruments while greatly improving sensitivity.