BioRT All-in-One RT Master Mix for qPCR（+gDNA Wiper）
This kit is a new-generation qPCR-specific reverse transcription kit. The product integrates all components required for genomic DNA removal and RNA reverse transcription into a single tube. Simply add template and water to initiate the reaction, which can be completed in as little as 5 minutes. The resulting cDNA is compatible with both dye-based and probe-based qPCR methods.
Product Features
All-in-One, Streamlined Workflow
– 4× All-in-One RT Mix in a single tube. Just one pipetting step, compatible with larger RNA input volumes.
Ultra-Fast Reaction, High Efficiency
– Complete over 90% genomic DNA removal and reverse transcription in as little as 5 minutes.
Versatile Priming
– Includes both oligo(dT)₁₀ and random primers for comprehensive RNA coverage.
Product Description
BioRT All-in-One RT Master Mix for qPCR (+gDNA Wiper) is a ready-to-use, single-tube reagent designed for fast and efficient reverse transcription of RNA into cDNA while simultaneously removing contaminating genomic DNA. The integrated gDNA Wiper step ensures high specificity and reliable downstream qPCR results without interference from residual DNA. With its optimized formulation, the mix supports both oligo(dT) and random primer priming, delivering high yields and full-length cDNA synthesis from various RNA templates. The streamlined workflow requires minimal pipetting, reduces handling errors, and can complete gDNA removal and reverse transcription in as little as 5 minutes, making it ideal for high-throughput and time-sensitive applications.
Application Field
Gene expression analysis
miRNA quantification
RNA virus detection
cDNA preparation for sequencing
Storage Conditions
1. The period of validity of this kit will not be affected by transportation under -25~8℃ condition for 7 days.
2. The kit should be stored at -25℃ ~ -15℃.
3. The kit can be stored for up to 12 months and please use the kit in the period of validity.
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