BioRT Master HiSensi cDNA First Strand Synthesis kit
This kit is designed for RNA reverse transcription experiments in the field of biochemical molecular research. It can convert various types of RNA (including total RNA, mRNA, tRNA, rRNA) into cDNA to meet the requirements for synthesizing cDNA of different lengths. The kit utilizes a modified Moloney Murine Leukemia Virus reverse transcriptase (M-MLV) with enhanced template affinity and reduced RNase H activity. This modification allows the enzyme to efficiently transcribe complex secondary structure templates and synthesize long cDNA fragments during the first-strand cDNA synthesis process. Additionally, it can reverse transcribe extremely low amounts of RNA templates (down to 1pg), significantly improving experimental efficiency.
Product Features
Efficient synthesis of first strand cDNA up to 14 kb.
Oligo (dT) and random primer are included with the kit.
30 min to complete cDNA synthesis.
A modified Moloney Murine Leukemia Virus reverse transcriptase (M-MLV) was added with enhanced template affinity and reduced RNase H activity.
Product Description
The kit is supplied with both oligo(dT)and random primer, In addition, customers can also use gene specific primers, random primers initiate cDNA synthesis at multiple random sites along RNA, ensuring unbiased representation. Oligo(dT) primers specifically prime cDNA synthesis from the poly (A) tail of mRNA, enabling selective amplification of mRNA transcripts. The benifits allows for unbiased initiation of cDNA synthesis from RNA, providing comprehensive coverage of the transcriptome and selective amplification of mRNA, enabling accurate analysis of gene expression.
Application Field
First strand cDNA synthesis for RT-PCR and RT-qPCR.
Construction of full length cDNA libraries.
Storage Conditions
-20 ± 5℃
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