BioRT One-step RT-qPCR Kit (UDG plus)
The reagent kit provided in this package offers a one-step reverse transcription (RT) real-time fluorescence quantitative PCR amplification reagent, enabling RT and PCR to be performed in the same reaction system. This reduces the risk of contamination during processes involving opening and transferring, among others. This product optimally integrates reverse transcriptase and antibody-modified hot-start Taq DNA polymerase, enhancing amplification sensitivity and specificity.
Product Description
The one-step reverse transcription (RT) real-time fluorescence quantitative PCR amplification reagent provided in this kit is used to make RT and PCR in the same reaction system, reducing the risk of contamination during operations such as opening the cap and pipetting. This product optimizes the integration of M-MLV (H-) reverse transcriptase and antibody-modified hot-start Taq DNA polymerase to improve amplification sensitivity and specificity. This product introduces the dUTP/UDG anti-contamination system. The heat-sensitive UDG can function at room temperature and can be inactivated when reverse transcribed at 50 °C, which can effectively prevent the risk of aerosol contamination of amplification products without interfering with the amplification reaction. At the same time, this product has optimized the reaction buffer system to promote the effective amplification of the template. Through the improvement of each component and the application of high-quality enzymes, the kit has the advantages of high amplification efficiency, strong specificity and high sensitivity.
Product Features
1. High Specificity: Utilizes a hot-start method to effectively control non specific reactions.
2. Contamination Prevention: The qPCR premix contains dUTP/UDG components to prevent false positives caused by PCR product contamination.
3. Wide Applicability: Suitable for probe-based assays.
4. Simple Operation: Few components make it easy to use.
5. Multiplexing: Supports multiple PCR reactions.
Application Cases
The PEDV control sample was subjected to gradient dilution using sample preservation solution, and then extracted using the MagaBio plus Virus Nucleic Acid Purification Kit III. The extracted material was subsequently used to compare and verify amplification performance and Ct values using the reagents from BSB73 and competing manufacturers N and T.
Results: The results show that in the amplification performance of PEDV using BioEasy's BioRT One-Step RT-qPCR Kit (containing UDG) with PEDV primer-probe detection, The amplification curves and Ct values of BSB73 are superior to those of the competitor products from foreign suppliers T and N.
Storage Conditions
1. The kit needs to be transported under frozen conditions.
2. Kit storage: Store in a -20°C refrigerator away from light, and the repeated freezing and thawing should not exceed 5 times.
3. Validity period: 12 months, please use within the validity period.
Ordering Information
Specification
Related Products
You May Also Like
BioRT All-in-One RT Master Mix for qPCR（+gDNA Wiper）
This kit is a new-generation qPCR-specific reverse transcription kit. The product integrates all components required for genomic DNA removal and RNA reverse transcription into a single tube. Simply add template and water to initiate the reaction, which can be completed in as little as 5 minutes. The resulting cDNA is compatible with both dye-based and probe-based qPCR methods.
BioRT Real Time RT-PCR Kit（SYBR Green）
The BioRT Real Time RT-PCR Kit (SYBR Green) is designed for efficient one-step fluorescent quantitative PCR detection using RNA as the template. This kit enables precise and reliable real-time monitoring of PCR amplification with SYBR Green dye, which binds specifically to double-stranded DNA. It offers a streamlined workflow, making it ideal for gene expression analysis, viral RNA detection, and other RNA-based research applications. The kit provides high sensitivity and specificity, ensuring accurate quantification of target genes with minimal preparation time.
BioEasy Universal qPCR Master Mix (SYBR Green)
This kit utilizes nucleic acid amplification technology combined with SYBR Green I fluorescent dye for Real-Time PCR, enabling rapid and accurate detection and quantification of target genes. The kit provides a 2× Universal SYBR Green Mix, requiring only the addition of primers and template to perform Real-Time PCR reactions, making it fast and easy to operate. Through improvements in the reaction components and the application of high-quality fast enzymes, this kit offers high amplification efficiency, strong specificity and fast reaction speed. The reaction and quantification efficiency for high GC sequences has been significantly enhanced. Additionally, the kit has optimized ROX dye in the system, allowing compatibility with various ROX versions of PCR instruments while greatly improving sensitivity.