HPV (6/11/16/18) Genotyping Real-Time PCR Kit (Fluorescent PCR)
This kit covers the types of HPV prevented by the quadrivalent vaccine (HPV 6/11/16/18). Specific primer probes were designed after selecting the conserved regions of HPV genome, and different fluorescence groups were labeled on the probes to produce different fluorescence in the reaction system. Four virus subtypes, HPV-6, 11, 16 and 18, can be classified and detected in only one detection, which can provide effective diagnostic basis for doctors and have guiding significance for diagnosis and treatment.
Product Features
1. There is no cross reaction between HPV-26、31、33、35、39、45、51、52、53、56、58、59、66、68、73、82、40、42、43、44、54、61、67、69、70、71、72、81 and 83 in this kit.
2. There was no cross-reaction with the human genome, ureaplasma urealyticum, chlamydia trachychondria, herpes
simplex virus TYPE II, human cytomegalovirus, treponema pallidum, Mycoplasma hominis, Neisseria gonorrhoeae,
Candida albicans, trichomonas vaginalis, Staphylococcus aureus, hepatitis B virus and hepatitis C virus.
3. At the same time, the presence of hemoglobin, white blood cells, cervical mucus, vaginal contraceptives, feminine
hygiene products, vaginal antifungal drugs and vaginal lubricants did not affect the testing of the samples.
Product Description
HPV (6/11/16/18) Genotyping Real-Time PCR Kit based on in vitro Real time PCR combining fluorescent probing. Primers and a sequence-specific fluorescence probes were designed tailored to Ll gene of HPV genome. Theprobes of HPV 18 are oligonucleotide attached fluorophores at the 5' end with FAM; the probes of HPV 16 are oligonucleotide attached fluorophores at the 5'end with HEX; The probes of HPV 6 are oligonucleotide attached fluorophores atthe 5' end with ROX; The probes of HPV 11 are oligonucleotide attached fluorophores at the 5' end with Cy5.5. In a meantime, specific primers and probeson basis of human B-Globin gene were developed as internal reference withfluorophores CY5 attached at 5’ end as reporter. All of probes 3’ end withquencher. During the PCR procedures, the DNA polymerase cleaves the probe atthe 5' end and separates the reporter dye from the quencher dye when the probes hybridize to the target DNA. This cleavage results in the fluorescent signal generated by the cleaved reporter dye, which is monitored real-time by the PCR detection system. Monitoring the fluorescence intensities during Real Time allowsthe qualitative detection of HPV (6/11/16/18) in specimens.
Application Field
HPV (6/11/16/18) Genotyping
Storage Conditions
It is recommended that specimens be processed as soon as possible after collection. If specimens are not processed immediately, they should bestored at 2-8 °C for up to 24 hours. If a delayed processing is expected, the specimens should be stored at -70°C or lower. Specimens should not be frozen and thawed frequently.
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