MagaBio plus Whole Blood Total RNA Purification Kit II
This RNA Extraction Kit is designed for rapid and efficient purification of high-quality RNA from 400 μL of whole blood in 45 min. The workflow is streamlined—after gentle mixing, whole blood samples can be directly loaded into the pre-filled reagent plates and processed on compatible automated instruments. The kit features a DNase treatment step to eliminate genomic DNA contamination, ensuring RNA purity and high yield. With excellent stability, simple operation, and high sensitivity, the purified RNA is completely compatible with downstream applications including real-time PCR, next-generation sequencing (NGS), and microarray analysis.
Product Description
This reagent kit releases RNA from blood samples under the action of Lysis Buffer. RNA specifically binds to magnetic beads, and the magnetic bead particles bound to RNA are captured by magnetic materials, and the DNA in the sample is digested by DNase Solution. Impurities are removed through washing processes with Wash Buffer. Finally, pure RNA are are eluted in Elution Buffer.
Product Features
Fast & Simple:
Easy-to-follow protocol; complete multi-sample purification in just 45 minutes.
Automated & High Throughput:
Enables 1 to 96 samples to be processed simultaneously with automated extraction instruments—simply load and start.
DNA-Free:
Integrated DNase digestion step ensures removal of residual DNA.
Safe & Eco-Friendly:
No use of toxic organic solvents.
Instrument Compatibility:
Fully compatible with Bioer GenePure Series Automatic Nucleic Acid Purification Instruments, NPAm-9600, and the FQD-A1600 Fully Automated Nucleic Acid Purification and Real-Time PCR Analysis System.
Application Field
Real-time PCR (qPCR):
For accurate and sensitive gene expression analysis.
Next-Generation Sequencing (NGS):
Suitable for transcriptome and RNA-Seq workflows.
Gene Expression Profiling:
Enables detection of differential gene expression in research and diagnostics.
Viral RNA Detection:
Ideal for molecular diagnosis of RNA viruses in clinical samples.
Storage Conditions
1.
Transportation conditions:
The DNase Solution is transported at 8℃ or below, and other reagent components are transported at room temperature.
2.
Storage conditions:
The DNase Solution is stored at -25℃ ~ -15℃, and other reagent components are stored at 2℃~30℃. Do not use beyond the expiration date printed on the box.
3.
Validity period:
According to the above method, it can be stored for 12 months.