pCaMV35S Gene Nucleic Acid Detection Kit (Fluorescence PCR)
With the rapid development of genetic engineering technology and its widespread application in biological improvement and genetic breeding, the variety of transgenic organisms and their products is gradually increasing. The Cauliflower mosaic virus (CaMV) 35S promoter is one of the most widely used promoters in genetic engineering and is present in various commercialized transgenic crops such as crops, corn, cotton, rapeseed, and tomatoes. Therefore, its safety assessment and detection research on transgenic crops are of great significance. This kit is suitable for detecting the transgenic component pCaMV35S gene in samples and is used to screen whether the sample to be tested contains the pCaMV35S promoter.
Product Features
Applicable Samples:
Crop plants and their processed products, herbivorous feed, etc.
High Accuracy:
Capable of effectively detecting the pCaMV35S gene with results meeting expectations.
High Sensitivity:
Can accurately detect the pCaMV35S gene in low concentration samples (1 pg/μL).
Simple Operation:
The amplification and detection process is completed entirely in a closed tube, effectively preventing aerosol contamination.
Product Description
This kit refers to the national standard to select the conserved region of the pCaMV35S gene sequence, and designs and synthesizes specific primers and a fluorescence-labeled Taqman probe. Compared to conventional PCR, this method offers higher automation, speed, sensitivity, and specificity. Additionally, the kit optimizes the reaction buffer system and the hot-start DNA polymerase, promoting efficient template amplification and reducing PCR run time. Furthermore, the product incorporates a dUTP/UDG anti-contamination system, effectively preventing the risk of aerosol contamination from amplified products. The aim is to establish a rapid fluorescent quantitative PCR detection method for the soybean Lectin endogenous gene.
Application Field
pCaMV35S Gene Nucleic Acid Detection
Storage Conditions
-20 ± 5°C, protected from light.