PEDV Real Time PCR Detection Kit
Porcine epidemic diarrhea virus (PEDV) belongs to the genus Alpha coronaviruses of the coronaviruses family. Porcine epidemic diarrhea (PED), a digestive or respiratory tract disease that is highly infectious and pathogenic in pigs, occurs frequently in the cold season of late winter and early spring, causing huge economic losses and seriously endangering the pig industry in China and the world. However, morbidity and mortality decreased with the increase of pig age. Suckling piglets had the highest infection rate and the highest mortality rate within 1-5 days of age, which was almost 100%. Weaned pigs, fattening pigs and breeding pigs had mild symptoms and very low mortality rate. The disease spreads rapidly, and within a constant number of days in pig farms, the whole herd is affected. Therefore, the diagnosis of this epidemic disease is very important.
Product Description
This kit designs primer probes in specific conserved areas of porcine epidemic diarrhea virus, and uses polymerase chain reaction (PCR) combined with Taqman fluorescence probe technology to achieve qualitative detection of pathogens, aiming to establish a fluorescence quantitative PCR detection method that can rapidly diagnose porcine rotavirus, and achieve the purpose of rapidly shunting pigs with related suspected symptoms, in order to effectively prevent and control swine rotavirus infection diseases.
Product Features
1. Applicable samples: pig intestinal contents, mesenteric lymph nodes, live pig excreta, etc.
2. Strong specificity: there was no cross-reaction with foot-mouth disease virus type O /A/Asian Type 1, porcine blue ear virus, porcine Rotavirus, porcine transmissible gastroenteritis virus, porcine Mycoplasma pneumoniae, porcine circovirus, porcine pseudo rabies virus, porcine parvovirus and classical classical swine fever virus.
3. High accuracy: can effectively identify porcine epidemic diarrhea virus, detection results in line with expectations.
4. Simple operation: complete the RT-PCR process in one step, complete closed tube amplification and detection, and prevent aerosol pollution.
Application Field
The kit is used for the detection of Porcine Epidemic Diarrhea Virus (PEDV) in intestinal contents, mesenteric lymph nodes, excreta of pigs. The kit for research only, cannot be used alone as the sole criteria for diagnosing or excluding cases.
Storage Conditions
1. The kit need to be transported under freezing conditions.
2. The kit should be stored at -25℃~-15℃ away from light, and avoid repeated freeze-thaw more than 5 times.
3. The kit can be stored for up to 12 months if all components are kept in the manner above. (Please use the kit in the period of validity).
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