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SARS-COV-2 Nucleic Acid Detection Kit (Fluorescence RT-PCR)

SARS-COV-2 Nucleic Acid Detection Kit (Fluorescence RT-PCR)

Respiratory Tract Infections Diseases

This kit is used for the qualitative detection of the SARS-CoV-2 nucleic acid in specimens of nasopharyngeal swabs, oropharyngeal swabs and sputum specimens from suspected cases. This kit selects the novel coronavirus 2019-nCoV Orf1ab and N gene fragments for primer and fluorescent probe design, and has excellent specificity. During the detection process, the human β-Globin gene is introduced as non-competitive internal control which can monitor the quality of the entire extraction and detection process to prevent invalid detection.

Product Description

This product selects the ORFlab (FAM) and N gene (HEX) regions of SARS-CoV-2, and designs three sets of primers and fluorescent probes. The three sets of primers and probes can specifically bind to the tanget sequences. Whenthe RT-PCR amplification reaction is performed, the fluorescent signal (s) can bedetected by a full-automatic fluorescent PCR detector to realize real-time online monitoring of the RT-PCR reaction. In order to control the entire extraction and detection process, human gene was act as a non-competitive internal control duringthe extraction and detection process.

Product Features

  • Duplex detection of ORF1ab and N genes

Enhances detection specificity and accuracy.

  • Efficient hot-start enzyme

Provides strong stability and reliable amplification performance.

  • Built-in internal control

Ensures result reliability and avoids false negatives.

  • High analytical sensitivity

Detects as low as 500 copies/mL of viral RNA.

Application Field

Respiratory infection disease detection

Storage Conditions

-25℃~-15℃

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